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首页> 外文OA文献 >Relationship between presynaptic membrane potential and acetylcholine release in synaptosomes from Torpedo electric organ.
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Relationship between presynaptic membrane potential and acetylcholine release in synaptosomes from Torpedo electric organ.

机译：鱼雷电器官突触体中突触前膜电位与乙酰胆碱释放之间的关系。

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摘要

The membrane potential of purely cholinergic synaptosomes isolated from Torpedo electric organ was monitored with fluorescent carbocyanine dyes. An increased fluorescence was associated with depolarization and a quenching with hyperpolarization. Fluorescence data provided evidence that Torpedo synaptosomes have a membrane potential mainly driven by a K+ diffusion potential and a membrane potential of about -50 mV could be estimated after calibration of fluorescence signals with ionophore antibiotics. The release of acetylcholine (ACh) from Torpedo synaptosomes was monitored continuously by measuring the light emitted by a chemiluminescent method (Israël & Lesbats, 1981 a). Using fluorescence data, the release of ACh was expressed as a function of membrane potential. The relationship between presynaptic potential and transmitter release as determined by biochemical methods at cholinergic nerve endings showed striking similarities to that observed at the squid giant synapse. Several substances were also tested with regard to their depolarizing and releasing properties and it was found that the toxin isolated from the venom of the annelid Glycera convoluta, which induced a large increase in quantal release of transmitter (Manaranche, Thieffry, & Israël, 1980) promoted a depolarization of Torpedo synaptosomes in addition to ACh release.
机译：从鸟雷电器官分离的纯胆碱能突触体的膜电位用荧光碳花青染料监测。荧光增加与去极化和超极化猝灭有关。荧光数据提供了证据，表明鱼雷突触小体的膜电位主要由K +扩散电位驱动，用离子载体抗生素校正荧光信号后，膜电位约为-50 mV。通过测量化学发光方法发出的光，连续监测鱼雷突触体中乙酰胆碱（ACh）的释放（Israël＆Lesbats，1981 a）。使用荧光数据，ACh的释放表示为膜电位的函数。通过生化方法在胆碱能神经末梢确定的突触前电位与递质释放之间的关系与在鱿鱼巨突触中观察到的惊人相似。还测试了几种物质的去极化和释放特性，发现该毒素是从无环线虫甘油的毒液中分离出来的，可导致递质定量释放的大量增加（Manaranche，Thieffry，＆Israël，1980）。除释放乙酰胆碱外，还促进了鱼雷突触体的去极化。

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    Meunier, F M;

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  • 年度 1984
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